Vaccinia Capping Enzyme

Catalog number: EN-1002

Introduction:

This product is the Vaccinia Capping Enzyme recombinantly expressed in E. coli. 

Vaccinia Capping Enzyme can add the 7-methylguanosine cap (Cap0) to the 5’ terminus of mRNA. This structure can improve the stability of mRNA, and is indispensable to subsequent transport and translation.

Properties:

Optimal reaction temperature: 37℃.

Definition of active unit: 1 active unit is defined as the amount of enzyme needed to incorporate 10 pmol of (α³²P) GTP into 80nt transcript within 1h at 37℃.

Quality control:

Purity ≥ 95%, residual host cell DNA ≤ 100pg/mg, residual host cell protein ≤ 50ppm, residual endotoxin ≤10EU/mg, no residual RNase, endonuclease, exonuclease or protease, germ-free, pathogen-free.

Capping rate as high as 95%; the 5'-Cap structure effectively promoting the in vivo expression of mRNA.